BIONICS Custom DNA/RNA/Aptamers/ Oligos

Custom DNA/RNA/Aptamers/ Oligos

Custom DNA Oligonucleotides

Tailored to Your Specifications

At Bionics, we offer a wide range of custom DNA oligonucleotides to meet your research needs. Our oligos can be tailored to your exact specifications, with options for modifications, scales, and lengths. With our high-quality synthesis and guaranteed yields, you can trust that your custom oligos will be delivered on time and to your exact specifications.

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At Bionics, we provide custom oligo pools that offer high fidelity, uniformity, low error rates, and low dropout rates, making them ideal for accurate and reliable CRISPR libraries, primer pools for multiplex PCR, gene construction, data storage, and FISH analysis.


  • High Quality: Our oligos come with ISO 9001 & ISO 13485 quality certifications, extremely low mutation, and low error rates, in accordance with the stringent quality control standards for oligo synthesis.
  • Good Stability: We ensure unrivalled control of oligo specifications that guarantee batch-to-batch consistency and traceability.
  • Highly Customizable: We offer flexible synthesis scales and four alternative purification options, including RPC, PAGE, and HPLC, to cater to our clients’ specific needs.
  • Technical Support: Our professional teams experienced in oligo synthesis and various modifications provide support throughout the entire production process.
  • Cost-Effective: We offer competitive prices and affordable services that do not compromise the quality of our products.


Our custom formatting capabilities have been optimized to provide complex formulations containing hundreds of oligos in a single pool, which can be provided in the required amount in ODs and supplied in dry format. Multiple aliquots of the oligo pool can be provided upon request. In addition to standard mixes, we specialize in preparing complex formulations containing varying amounts per oligo within the pool. Simple oligo mixes can be provided in plates or tubes, and complex formulations containing a large number of oligos will be provided in the appropriate tube based on the pool volume or the customers required format.


In addition to our custom oligo pools, we also offer specialized oligos, including NGS oligos, peptide-oligonucleotide conjugates, complex oligos, large-scale oligos, and oligo aptamers, to cater to various research needs.

Unmodified DNA Oligo synthesis:
Length Purification Method
11-59nt Final Yield (5 OD) Final Yield (2 OD) Final Yield (2 OD) Final Yield (50 OD)
50 nmol
100 nmol 2 OD 2 OD 5 OD
200 nmol 5 OD 5 OD 10 OD
1 μmol 50 OD 10 OD 10 OD
60-90nt Final Yield (5 OD) Final Yield (2 OD) Final Yield (2 OD) Final Yield (50 OD)
50 nmol
100 nmol 2 OD 2 OD 5 OD
200 nmol 5 OD 5 OD

Custom Synthesis Scales

We understand that every project is unique. That’s why we offer custom synthesis scales and lengths to suit your specific needs. Please don’t hesitate to contact us for a quote.

Please Note:

  • The scale in nmol refers to the starting material for synthesis, not the final yield of the oligo.
  • The final yield is always measured in OD.
  • We understand that determining the appropriate amount of oligos needed for your assays can be challenging, and selecting the correct synthesis scale can be even more complex.

PCR Primer Quantity Calculator

How many reactions can you do with 25 nmol PCR primers?

To determine how many PCR reactions you can do with 25 nmol PCR primers, consider the following:

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  • Most PCR reactions use 0.1−0.5 μM primer. Assuming a maximum concentration of 0.5 μM and a reaction volume of 20 μL, each reaction will require 10 pmol oligonucleotide primer.
  • For a typical 25mer oligonucleotide, 1 OD is equivalent to approximately 4 nmol, or 4000 pmol. Guaranteed yield for a 20 base PCR primer on the 25 nmol scale is 12 nmol.
  • Therefore, with even the minimum yield from a 25 nmol synthesis, you should be able to perform 1200 PCR reactions.

In summary: minimum guarantee for a 20mer ≈ 12 nmol = 12,000 pmol = 1200 reactions.

Custom DNA Oligos with Modifications

Bionics offers a wide range of custom DNA oligos with modifications to meet your research needs. Our modifications include, but are not limited to:

  • Fluorescent dyes and quenchers
  • Phosphorothioates
  • Biotin
  • And many more!

Contact us today to request a quote or to learn more about our custom DNA oligo services.

Custom DNA Oligos Modifications
Fluorescent Dyes Labels
The most important fluorescent compounds used to label oligonucleotides are fluorescein and various fluorescein analogs. Fluorescein is a multi-ring aromatic compound that is strongly fluorescent. * The fluorescent color was calculated with the maximum emission wavelength, for your reference only.
Label Name Purifications Positions Excitation Maximum (nm) Emission Maximum (nm)
Cy3 HPLC 5′, 3’End 549 566
Cy5 HPLC 5′, 3’End 646 669
FAM HPLC 5′, 3’End 495 520
HEX HPLC 5’End 535 556
TET HPLC 5’End 521 536
FITC HPLC 5′, 3’End 492 515
6-JOE HPLC 5′, 3’End 529 555
ROX HPLC 5′, 3’End 586 610
TAMRA HPLC 5′, 3’End 557 583
Helix Fluor555 HPLC 5′, 3’End 542 558
Helix Fluor575 HPLC 5′, 3’End 546 575
Texas Red HPLC 5′, 3’End 588 601
Quasar 570 HPLC 5′, 3’End 548 566
Quasar 670 HPLC 5′, 3’End 647 670
Cy3.5 HPLC 5′, 3’End 581 596
Cy5.5NS HPLC 5′, 3’End 678 701
Cy7NS HPLC 5′, 3’End 750 780
CAL Fluor Red 590 HPLC 5′, 3’End 569 591
CAL Fluor Red 610 HPLC 5′, 3’End 590 610
DABCYL HPLC 5′, 3’End 453 0
AMCA HPLC 5′, 3’End 353 455
DEAC HPLC 5′, 3’End 411 471
MCA HPLC 5′, 3’End 322 390
LRB Red HPLC 5′, 3’End 568 583
CR6G HPLC 5′, 3’End 524 556
Bodi Fluor R6G HPLC 5′, 3’End 528 547
NBD-X HPLC 5′, 3’End 467 539
California Red HPLC 5′, 3’End 583 603
iFluor™ 647 HPLC 5′, 3’End 649 664
iFluor™ 660 HPLC 5′, 3’End 662 678
iFluor™ 680 HPLC 5′, 3’End 676 695
iFluor™ 700 HPLC 5′, 3’End 685 710
iFluor™ 710 HPLC 5′, 3’End 712 736
iFluor™ 750 HPLC 5′, 3’End 749 775
iFluor™ 800 HPLC 5′, 3’End 801 820
VIC HPLC 5’End 538 554
LC RED-610 HPLC 3’End 590 610
LC RED-640 HPLC 3’End 625 640
LC RED-705 HPLC 3’End 685 705
Bodipy 493/503 HPLC 3’End 493 503
Bodipy 564/570 HPLC 3’End 564 570
Bodipy 581/591 HPLC 3’End 581 591
Bodipy 630/650 HPLC 3’End 630 650
Bodipy 650/665 HPLC 3’End 650 665
BODIPY R6G HPLC 3’End 528 547
SIMA HPLC 5’End 533 557
Yakima Yellow Epoch HPLC 5′, 3’End 530 549
Dual Labels
Dual-labeled fluorescent probes typically contain a 5′ fluorophore and a 3′ quencher. The probes are often designed to anneal between the upstream and the downstream primer in a PCR reaction. These are commonly used with quantitative PCR, mutation detection, allele determination, and SNP detection.
Label Name Purifications Positions
5’FAM-3’TAMRA HPLC 5′, 3’End
5’HEX-3’TAMRA HPLC 5′, 3’End
5’TET-3’TAMRA HPLC 5′, 3’End
5’JOE-3’TAMRA HPLC 5′, 3’End
5’VIC-3’TAMRA HPLC 5′, 3’End
5’FAM-3’BHQ1 HPLC 5′, 3’End
5’VIC-3’BHQ1 HPLC 5′, 3’End
5’HEX-3’BHQ1 HPLC 5′, 3’End
5’JOE-3’BHQ1 HPLC 5′, 3’End
5’TET-3’BHQ-1 HPLC 5′, 3’End
5’Cy5-3’BHQ2 HPLC 5′, 3’End
5’Cy3-3’BHQ2 HPLC 5′, 3’End
5’ROX-3’BHQ2 HPLC 5′, 3’End
5’TET-3’BHQ2 HPLC 5′, 3’End
5’JOE-3’BHQ2 HPLC 5′, 3’End
5’HEX-3’BHQ2 HPLC 5′, 3’End
5’TAMRA-3’BHQ2 HPLC 5′, 3’End
5’Texas Red-3’BHQ2 HPLC 5′, 3’End
5’Quasar 670-3’BHQ2 HPLC 5′, 3’End
5′-FAM and 3′-BHQ2 HPLC 5′, 3’End
5’Quasar 670-3’BHQ3 HPLC 5′, 3’End
5’CY5-3’BHQ3 HPLC 5′, 3’End
5’FAM-3’ECLIPS HPLC 5′, 3’End
5’HEX-3′ ECLIPS HPLC 5′, 3’End
5’TAMRA-3’Eclipse HPLC 5′, 3’End
5’ROX-3’Eclipse HPLC 5′, 3’End
5’TET-3’Eclipse HPLC 5′, 3’End
5’JOE-3’Eclipse HPLC 5′, 3’End
5’FAM-3’MGB HPLC 5′, 3’End
5’HEX-3’MGB HPLC 5′, 3’End
5’TET-3’MGB HPLC 5′, 3’End
5’JOE-3’MGB HPLC 5′, 3’End
5’ROX-3’MGB HPLC 5′, 3’End
5’Texas Red-3’MGB HPLC 5′, 3’End
5’Quasar 670-3’MGB HPLC 5′, 3’End
5’VIC-3’MGB HPLC 5′, 3’End
5’FAM-3’DABCYL HPLC 5′, 3’End
5’HEX-3’DABCYL HPLC 5′, 3’End
5’TET-3’DABCYL HPLC 5′, 3’End
5’JOE-3’DABCYL HPLC 5′, 3’End
5’Texas Red-3’Dabcyl HPLC 5′, 3’End
5’ROX-3’Dabcyl HPLC 5′, 3’End
5’CY3-3’DABCYL HPLC 5′, 3’End
5’Cy5-3’DABCYL HPLC 5′, 3’End
5’Phos and 3’FAM HPLC 5′, 3’End
5’TAMRA and 3’Phos HPLC 5′, 3’End
5’C6-Biotin,3’Biotin HPLC 5′, 3’End
5’C6-Biotin,3’Cy5 HPLC 5′, 3’End
5’C6-NH2,3’C3-Fam HPLC 5′, 3’End
5’C6-NH2,3’Cy3 HPLC 5′, 3’End
5’Cy5,SS, HPLC 5′, 3’End
5’Cy5,3’C7-NH2 HPLC 5′, 3’End
5’Cy5,3’SH HPLC 5′, 3’End
5’Dabcyl,3’C3-Fam HPLC 5′, 3’End
5’DlG,3’Digoxin HPLC 5′, 3’End
5’SH,3’Cy3 HPLC 5′, 3’End
5’Fam,3’C3-Fam HPLC 5′, 3’End
5’TAMRA,3’C3-Fam HPLC 5′, 3’End
FRET quenching depends on the ability of the fluorophore to transfer energy to the quencher. For this to happen, the emission spectrum of the fluorophore must overlap with the absorption spectrum of the quencher. For a quencher to quench fluorescence from several different fluorophores, it must therefore have a wide absorption spectrum and a high extinction coefficient.
Label Name Purifications Positions
BHQ 1 HPLC 3’End
BHQ 2 HPLC 3’End
BHQ 3 HPLC 3’End
BBQ-650 HPLC 3’End
TQ1 HPLC 3’End
TQ2 HPLC 3’End
TQ3 HPLC 3’End
TQ4 HPLC 3’End
TQ5 HPLC 3’End
TQ6 HPLC 3’End
QSY 7 carboxylic acid HPLC 3’End
TQ7 HPLC 3’End
Affinity Ligands:
Oligo nucleotides can be labeled with biotin or digoxigenin or directly labeled with alkaline phosphatase. A variety of linker arms are available as spacers to minimize steric hindrance.
Label Name Purifications Positions
Biotin dT HPLC 5′, 3’End, Internal
Biotin HPLC 5′, 3’End, Internal
Biotin-TEG HPLC 5′, 3’End, Internal
DIG HPLC 5′, 3’End
Spacer modifications C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. These modifications can be added in multiple additions when a longer spacer is required.
Label Name Purifications Positions
Spacer C6 HPLC 5′, 3’End, Internal
Spacer 9 HPLC 5′, 3’End, Internal
Spacer C12 HPLC 5′, 3’End, Internal
Spacer C3 HPLC 5′, 3’End, Internal
Spacer C18 HPLC 5′, 3’End, Internal
D Spacer HPLC 5′, 3’End, Internal
Attachment moieties:
A primary amino group or a thiol group can be used to attach a variety of modifiers (such as fluorescent dyes) to an oligonucleotide or used to attach an oligonucleotide to a solid surface.
Label Name Purifications Positions
5’-Amino-Modifier-C6 HPLC 5’End
3’-Amino-Modifier-C7 HPLC 3’End
5’-Amino-Modifier-C12 HPLC 5’End
Thiol-Modifier C6 S-S HPLC 5′, 3’End, Internal
Thiol-Modifier-C3 S-S HPLC 5′, 3’End, Internal
TM increase
The stability of the DNA double helix depends on a fine balance of interactions including hydrogen bonds between bases, hydrogen bonds between bases and surrounding water molecules, and base-stacking interactions between adjacent bases. Slight variations in the DNA sequence can have profound implications on the stability of the DNA duplex.
Label Name Purifications Positions
2’ OMe-rA PAGE 5′, 3’End, Internal
2’ OMe-rC PAGE 5′, 3’End, Internal
2’ OMe-rG PAGE 5′, 3’End, Internal
2’ OMe-rU PAGE 5′, 3’End, Internal